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The increasing use of powder metallurgy techniques to make an almost infinite variety of materials and products places greater emphasis on utilization of sophisticated experimental techniques. Usually research and development efforts initiate the use of newly developed equipment and analytical procedures. Indeed, the contents of this book are strongly linked to research endeavors, in both the academic and industrials worlds. However, this volume can serve a much needed function in industrial applied powder metallurgy. Although many researchÂ ers will find the contents of great value, the technical personnel more involved with production, quality control, customer services and product design now have at their dispoÂ sal a means to learn about the potential uses of several very important techniques. With today's "knowledge explosion" the present set of papers greatly facilitates the comprehension and adoption of new procedures. If powder metallurgy is to continue its rapid rate of growth in virtually all segments of industry, then the transition of modern equipment and procedures from tools of research and development laboratories to everyday plant operations and applications must be hastened. The editors hope that this volume aids in this process, as well as assisting students and researchers by providing a ready source of up-to-date useful information.
For Jeff Brennan, juggling multiple identities is a way of life. Online he has dozens of different personalities and switches easily between them. Offline, he shows different faces to different people: the caring grandson, the angry ecoprotester, the bored IT consultant. So when the beautiful Marie mistakes him for a famous blogger, he thinks nothing of adding this new identity to his repertoire. But as they fall in love and start building a life together, Jeff is gradually forced into more and more desperate measures to maintain his new identity, and the boundaries between his carefully segregated personas begin to fray. In a world where truth is a matter of perspective and identities are interchangeable, Jeff finds himself trapped in his own web of lies. How far will he go to maintain his secrets? Even if he wanted to turn back, would he be able to?
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
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